Single cell transcriptomics identify AHR and CD9 as markers of CD14 +atypical B cells

Abstract B lymphocytes are adaptive immune cells responsible for the production of antigen specific antibodies and release pro- anti-inflammatory cytokines. Pro-inflammatory cells, often termed atypical (at cell) a heterogenous population associated with aging, infection, autoimmunity. Aryl hydrocarbon receptor (AHR) is ligand-activated transcription factor important xenobiotic metabolism. However, AHR heavily implicated in cell differentiation as regulator fate decisions. at believed to represent an alternative lineage but role not known. We have reported that human, primary CD5 +innate-like (ILB) preferentially sensitive modulation by AHR-activation compared −B cells. +ILB enriched IgM memory B1 marginal zone regulatory used Single transcriptomics elucidate expression human naïve, circulating CD19 +B isolated directly from PBMC protein. found correlated CD14and were myeloid gene signature. These results validated CD9 PBMC. Further, CD14 expressing expressed higher CD11c Tbet lower IgD, markers data suggest putative +at (Supported NIH grant P42ES004911) Supported P42ES004911